Status:RUO Cat No.:661391. 3� �J�`54�`?�T-&���gp M�{�G��6�U�*��~u����Fߜ�@.��HY~���^�l*k��S��3c�rɝ�å�NW��ĩ>>9x���y�_�m�Z�tf���>����%�,�+��t��,>:.��/ ��檪�s;Ł����$��m�i5��)|�ʽ�ɖ�GY�(�Dv.�&���a���� 8����C}r�}���0�t�X�.��-MԬ��i��e��d�f�R? Accuri C6 is a 4 color (2 lasers) instrument, Facs Verse may be up to 8 (3 lasers) color. I played around with WinMDI but something weird happend when I comparing the data analysed in cell quest. I used both 2-laser Calibur and 3-laser cantoII. Inquire. If you’re new to flow cytometry, and/or use one of the modern benchtop flow cytometers which don’t require, or even allow, you to mess with the guts of the instrument, you may not care much about how flow cytometers work. 27. Update Driver Software - Cytometer, Second Dialog Box. Preventative Maintenance on the BD Accuri™ C6 Plus - YouTube It is to hard to see samples during running of sds-page. Windows Security Window . Dear Ractim. In the US, contact: traininginfo@bd.com or call 1.877.232.8995, prompt 5. It also includes common troubleshooting … What are your thoughts? Each with lock and regular washer a. 6. CONVENIENCE IN YOUR LAB. As with anything new there were a few bugs and the software takes a bit of getting used to. It also includes common troubleshooting help and service information. It is a very user friendly machine and has very little maintenance issues. December 2017 BD Accuri C6 Flow Cytometer Repair Brad Elmore Before working on instrument. I have used FACScaliber. The separation layers now visible. I study few protocols, somewhere they said i need to fix it with PBS and when i used than i will need to stain with antibody. The C6 was designed to be a cheap instrument, and lives up to its expectations. In other words, if you think to use multicolor (ie 5-8 color) protocols, obviously the Verse is the only possible choice. Originally we were going to purchase the FACsCanto II however the reps mentioned the recently available FACsCelesta. As with most instrument purchasing decisions, I highly recommend you run some of your samples with your panels of antibodies on both instrument to generate some real-world data and decide for yourself. Figure 5-3. It is by no means a poor instrument, it's just not very good. We have had our Accuri since 2008 and it has been great. BD CSampler™ Plus CSampler Plus:24-tube rack and 96-well plate automated sample loader for BD Accuri C6 Plus Flow Cytometer. How many of you have problems with the Accuri Valves ? Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. %PDF-1.6 %���� The Facsverse is a much more complex machine doing up to eight colors with 3 lasers. h��X�n�8����[:��E��I�L�6Swôy`d��T�#�mү�{IѲU�΂�� ��]�����I�0�e�IB|���Ы���O�w���.ZY�x�#���BF���jb�{Q����a��.sx�c>��9V�x{'c�J䍄�3xSՅ�a�縿3x{�Zޞ�9.��U/$��D� ]K �o�oԫ�JB*t\57q/_�̯�?=9��M*K�$�l:SN��0��z,�Sh�"�0�uVM৬+���GjVK W�w Mv:�`Fp G� Can someone give some suggestion for the choice of maker? The BD Accuri C6 has a 1-minute UNCLOG cycle which may be run any time an obstruction is suspected. The BD Accuri C6 Plus offers plug-and-play simplicity and walk-away capabilities thanks to turnkey, automated software. ]��E�z,�&l��� �9w�@7) ],m� 98 0 obj <>/Filter/FlateDecode/ID[<00FCDA4AADBD4BAE9B9E563DAC7F2050><401A5750BC3C5B448D6FC8BE2B4CA82B>]/Index[73 40]/Info 72 0 R/Length 112/Prev 808970/Root 74 0 R/Size 113/Type/XRef/W[1 2 1]>>stream 3 on bottom. From basic to more challenging applications, the BD Accuri™ C6 Plus personal flow cytometer is a powerful benchtop tool that provides you with the versatility and the analytical power of a cytometer twice its size. I also have experience with the Accuri. The Accuri C6 cytometer in my lab has some problems, and we need some help. Our machine is still sitting in San Jose at BD over a year later because we haven't decided if it's worth paying for the repairs. But I think having a machine with more channels is better as it allow you to look at different stuffs on precious samples. VERSATILE. In addition, the fluidics take a unique perspective on moving fluid; utilizing a vacuum system to pull fluid through the flow cell instead of a pressure system to push fluid throughout. *D������X[��K|�Zu�yM��п^�@�>&�A. 0 How you prepare 6x laemli buffer with mercaptoethanol? A month later, we have (again) a problem with the valves. I want to identify neutrophil in rat lung tissue by immunohistochemistry. What is the best? The BD Accuri C6 Plus only weighs only 30 pounds and has a compact footprint of 11 x 14.75 x 16.5 inches, but empowers a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. I have used a few different flowcytometers and the facsverse is easily the best machine out of the lot. Thank you very much. BD Accuri™ C6 Plus Workstation Computer and Software. It is simple to use, inexpensive, and easy to train novices on. Can anyone recommend a good free FACS analysing software? see your device’s user manual. Align the front of the BD CSampler so that the holes on the module are lined up with the three threaded holes in the BD Accuri C6. One more thing, Is accuri generated files supported by any of windows based software? I disagree with the prior comments. Another very important issue is the amount of events you think you have to acquire for each tube. 11. Introducing the BD Accuri C6 Plus. I have tried to isolate PBMC from the whole blood using ficoll solution and so far, I have followed all the procedure but the result wasnt as I expected. Software options … RELIABLE. So the choice should be addressed by the kind of flow experiments you plan to do. 1) Open instrument 2) Remove hex bolts holding instrument guts in chasis. The two instruments are not really comparable. Manuals and User Guides for BD Accuri C6. h�b```����� ce`a�X�8�(�7qA����F��#�C��D��4x.JMJ����N2CT� /��Y/܂w���o�^(��P�B��u-�6-�[/���[�$e���Mo�x;��DY��G[�M�XAGȀp �mBGn��yEG�Ky�b4S��hxEGG��4 $Τ(������W���g> -�r �0�2���e|%�ѫi���<7��^R���H�*�Ǯ���F! It is a great machine to train students on. A lot of highly competent molecular biologists, immunologists, etc., look at the flow cytometer as a tool, and just want to see... Join ResearchGate to find the people and research you need to help your work. For walkaway convenience, the optional BD CSampler™ accessory offers reliable and easy-to-use automation. Yes Accuri files are FCS3.0 so compatible with all programs that have been updated to accept the expanded range for all parameters. I have a BD Accuri C6 in my lab for four years, and met different kinds of problems, such as the valve problem a lots of people have been talking. We sent the Accuri C6 Plus (received new Nov 2016 and this issue happened July 2018) into BD to be properly diagnosed and find out the cost for repair. It is an excellent basic machine (4 color) with a large dynamic range for analysis, allowing the measurement of both very small and very large cells. The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. The downside to the FACSVerse is that it had some issues when it first came to market, but I believe many of those issues have been ironed-out. Browse for Folder Dialog Box. One of the main advantages of this is the ability to use a variety of sample tubes/plates from which to collect samples. To me BD FACSVerse is better than BD acuuri. %%EOF I want to store my cells, and want to run FACS after few days. Has anyone demoed or purchased this machine? A: BD Accuri C6 training and applications support at customer sites will be offered based on the availability of staff with product knowledge. INFO BELOW ... SB3820-C6 Model Information - Vizio As always, we want to … so which is the better option, i could just wash with PBS and store it. On the other hand, if you need flow cytometer only for simple and repetitive experiments C6 might be enough. BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express™ software and FlowJo™ software. Please contact your regional customer-training center for more information. Planning to have a flowcytometer. FCS 2 (Verse) is not compatible with free software like Flowing 2 etc. The system supports 48- and 96-well plates and deep- well plates, and is also supplied … Status:RUO Cat No.:660519. If you want to know more about the Accuri you can contact me. and in case, if i need to stain with antibody, than i could stain with it for 30 mints only or i need to stain it with antibody till i used it ???? http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cell-Analysis/Flow-Cytometry/Flow-Cytometers/Attune-Acoustic-Focusing-Flow-Cytometer.html?CID=fl-attune, The optimisation and use of multilaser, multiparameter flow cytometers for polychromatic flow cytometry, How Flow Cytometers Work — and Don’t Work, The limits of calibration in flow cytometry: Flow cytometers are not strictly linear. Direct counts on the BD Accuri C6 correlate highly with counting beads, and are more precise than hemocytometer counts. I'm completely agree with the words of Ryan Duggan. h�bbd``b`��@�A��$�v �_ �1�]"f��GA�$Xށ�Wd +�`)�����:����@��rw�n��e`$��ϴ�@� j�Z The Accuri C6 cytometer in my lab has some problems, and we need some help. Are there anyone has different protocol working good? Quick View . The FACSVerse is a far superior instrument to the C6. 3) Remove back plate. also are there other programs out there? I am analysing my data with cell quest (which is not my favorite program) I used to use Flowjo (which rocks in my opinion) but have no means to ever get this. A BD Biosciences technical bulletin contains recommendations, tips, techniques, and troubleshooting suggestions to help maximize the accuracy of cell counts and concentrations on the BD Accuri C6. Just few consideration more. It needs not special tubes (you can use eppendorf cups, 15 ml tubes, plates..). We sent the Accuri C6 Plus (received new Nov 2016 and this issue happened July 2018) into BD to be properly diagnosed and find out the cost for repair. To be honest with you although the Accuri C6 has still some minor issues that could be sorted out (and I´m sure they´re working on it), even with those problems, I love it, as I said before it´s great for small groups and labs, but also a great complement for a core facility. This protocol also assumes a completed assay in a 96-well plate, with particles suspended in Run buffer. We have 1 BD Accuri C6 manual available for free PDF download: Instrument Manual ... Troubleshooting and Technical Support. Use a Wrist Grounding Strap. Can anyone please suggest one which will be the best choice between BD acuuri C6 or BD FACSVerse? I was using Mendeley and thinking of moving to Endnote as majority of researchers using it. Any thoughts on the FACsCelesta flow cytometer compared with the FACsCanto II? Hold the module with one hand and screw in the front right mounting bolt to secure the BD CSampler to the BD Accuri C6, tightening with the tool provided (Figure 6). The FACSVerse was designed to showcase the next generation of BD engineering, and although that comes with a few growing pains, it really does allow for some very nice advantages. 73 0 obj <> endobj The basic problem of BD acuuri that you can not adjust the PMT voltage as per your experiments and cells, at least in case of instruments what BD are selling in India. There is nothing like the Verse. Still I would love to know the better one. What is the best marker for identification of neutrophil in lung tissue by immunohistochemistry? So be sure that you do not need to change the PMT voltage. 1.1 About This Manual This manual provides information on cytometer requirements and specifications, setup, and maintenance. Place an empty tube on the sample port and select the UNCLOG button in the workspace. For information on installing and using the BD CSampler™, see the BD Accuri BD Accuri™ C6 Flow Cytometer Instrument Manual system, etc.) 26. All rights reserved. Sort by: Instrument Options (1 of 1) results found. However, this comes with the cost as well, not only at purchase but also with the maintenance. I was preparing 6x laemli buffer with mercaptoethanol as giwen below: Before use add 1/8th volume of β-mercaptoethanol. we have figured it out of how to solve the problems with BD Accuri C6 flow cytometer. The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. So the choice should be addressed by the kind of flow experiments you plan to do. However, when I used it with protein sample, its color was so light. Mendeley or Endnote as a reference manager software? Add the whole blood to conical tube that contain 4 ml of PBS (equal volume to the sample; 1:1), Add the entire solution to a new conical tube containing 4ml ficoll solution, Carefully & slowly layer the blood mix over the ficoll. The unusually high dynamic range of the ADC allows simultaneous analysis of species for which C-values span almost the entire range of the described angiosperms. Upper level (top of guts), 3 total: right, front, and back b. The Accuri is an excellent machine. Remove and discard the black SIP cover and place a tube containing at least 2mL of filtered, de-ionized water on the SIP. Ospedale Ordine Mauriziano di Torino, Umberto I. The BD Accuri C6 Software installer is on the CD that came with the C6. The cycle may be repeated until fluid is observed in the tube. 27. Always protect the Sample Introduction Probe (SIP) with a tube of HPLC reagent grade filtered, deionized H 2 O (0.22 µm filter), or equivalent, when the cytometer is not running samples. BD Accuri C6 cytometer simplifies system maintenance with automatic cleaning cycles on instrument shutdown. I was wondering if we were the only ones with this type of problem. Good pint added by Wiener. 1. Figure 5-2. I'm now searching makers to label monocyte for flow analysis, and also sorting. Our machine is still sitting in San Jose at BD over a year later because we haven't decided if it's worth paying for the repairs. BD Accuri C6 / plus -- Problem again and again? BD Accuri C6 with plate handler acquisition April 2016 Multi-well Auto Collect sample Important – only run this protocol after you have performed “Cytometer Setup/Verification” protocol on your BD Accuri C6 cytometer using particles. The system can employ laboratory-grade water for sheath fluid, reducing operating costs. The software is complicated but Diva (the software of Canto II) was any better...You can also determine the concentration of your sample without calibration beads or calibrate fluorescece/compensate it is not necessary anymore (it is automatically done by cheking Quality Control with dedicated beads, that you have to use once a day). Thank you all for your valuable comments and suggestions. If you have any questions feel free to Comment below! After spinning, remove carefully the conical tube. Our Accuri had different problems just before Christmas and BD changed a whole series of parts, one of them was valves. In addition to the excellent comments above, the decision has to be made based on what you to do (number of parameters to investigate at once, cell type of interest etc...). Figure 5-4. Even new flow cytometry users find it so easy to learn and use that most can collect and analyze data in less than an hour. I'm a new comer of the immunology field. It depends on what you want to do. The C6 uses two lasers, red and blue, to record events and has 4 fluorescence channels for a wide range of fluorescence detection. I have had an Accuri for 4 years and love it. however, somewhere i read that i need to wash with PBS, stain with antibody for 30 mints and than wash with PBS to store. Medical Research Council Unit, The Gambia Unit. I used PBMC activated with pokeweed and stained with anti IFNg (intracellular -657) and CD4-FITC. Has anyone already tried ? According to protocols, I will collect the cells and wash in PBS, than i will stain with a antibody (Fab fragment antibody) for flow cytometry. C6 User Guide BD Accuri™ C6 Flow Cytometer Instrument Manual NOKIA C6-00 USER MANUAL Pdf Download. What's the best maker for monocyte/macrophage in mouse peripheral blood? The BD Accuri™ C6 flow cytometer is ideally suited for determining C-values for new plant species. Gently homogenize the blood sample inside heparin blood collection tube. Our lab is looking to purchase a flow cytometer. Professors can request their students to be trained on the Accuri Student Cytometer by sending an email to Sandra Hope. endstream endobj 74 0 obj <>>> endobj 75 0 obj <> endobj 76 0 obj <>/Font<>/ProcSet[/PDF/Text]/XObject<>>>/Rotate 0/TrimBox[0.0 0.0 612.0 792.0]/Type/Page>> endobj 77 0 obj <>stream I also like to ground instrument. SIMPLE. It also includes common troubleshooting help and service information. To install BD Accuri C6 Software, run BDAccuriC6SoftwareInstaller.exe. It will be helpful if the user interface is similar to the Cell quest. Figure 5-1. I am using cancer cell lines for my study. I infected the 293tt cell with AAV virus to check the expression of my CAR molecule. or i also need to stain it with Ab and than store it ? Fluid should accumulate in the tube. © 2008-2020 ResearchGate GmbH. Unplug. 112 0 obj <>stream Such counts are essential in many research and clinical applications, including enumerating leucocytes, B cells, T cells, and platelets in human blood, measuring of cultured cell lines. The BD Accuri C6 Plus personal flow cytometer is the newest generation of the BD Accuri platform. But I think neutrophils also express CD11b and Ly6c. BD Accuri C6 Plus software features an intuitive user interface. Photoshop User Guide - Adobe Inc. C6 Corvette Buyers Guide / Review will inform you of the not so obvious changes through the life of the C6 Corvette (2005-2013). How to stain and fix cell for flow cytometry for long time ?? In this episode, I present to you, maybe the best entry level flow cytometer in the market, BD Accuri C6. some of the percentages of population rose from ca 0.5% (cell quest) to around 20% (WinMDI) any ideas? I have good experience with this. (pic 2), Hope you guys can help me with this. By looking excitation spectra I would say no (~5% excitation), but its maybe enough in practice ? What is the best marker for neutrophil identification by immunohistochemistry? (Pic 1), Load the conical tube without disturbing the layer. Accuri Cytometers ii 7820041-01 Rev-A 4.12.2 Correcting Fluorescence Spillover ..... 55 Accuri C6 is a 4 color (2 lasers) instrument, Facs Verse may be up to 8 (3 lasers) color. Quick View. Verse is able to acquire and process millions of events, C6 do not. The Accuri C6 Flow Cytometer does not produce aerosols during normal operation. What is the best way to isolate PBMC from whole blood? You may also check the Attune Acoustics Focusing Cytometer (, We have a 3 laser Verse with automatic sample. I recommend you BD FACSVerse. The BD company is very serious, with a good service. I got poor separation layer and I couldnt isolate the pbmc layer properly because of this. Introduction The BD Accuri® C6 personal flow cytometer can simplify cell analysis by counting absolute cell numbers directly (per unit sample volume). endstream endobj startxref 26. The BD Accuri C6 Plus personal flow cytometer. For information on using BD Accuri C6 Software, see the BD Accuri C6 Software User Guide. It uses a bunch of new technology that BD has built to address usability (software) and reliability (hardware). This manual provides information on cytometer requirements and specifications, setup, and maintenance. $25,000.00. The key features of the FACSVerse include a high level of optical filter quality assurance, such that the software can actually predict (with decent accuracy) the amount of compensation needed to correct for spectral overlap. The BD Accuri C6 flow cytometer is easy to use and allows student access to the science of flow cytometry. N {����>�'p )L �` The major drawback is in its software, but I export files and analyze on FlowJo. BD Accuri C6 Software User Guide - Manual 7820095-01 Rev-0 i TABLE OF CONTENTS 1 INTRODUCTION TO BD ACCURI C6 SOFTWARE.....1 I have been doing flow for over 25 years and used many machines and actually found the Accuri to be far superior for working with large cells such as macrophages. Was so light Manual provides information on using BD Accuri C6 cytometer in the workspace discard the black cover! Rack and 96-well plate automated sample loader for BD Accuri C6 Plus plug-and-play. A: BD Accuri C6 Plus - YouTube Introducing the BD Accuri C6 Plus - YouTube Introducing the BD C6! Maker for monocyte/macrophage in mouse peripheral blood of this Facs analysing software was using Mendeley and thinking moving. Installer is on the SIP for new plant species software installer is the! Thoughts on the BD Accuri™ C6 flow cytometer Repair Brad Elmore Before working on.... Use a variety of sample tubes/plates from which to collect samples sample for... Think having a machine with more channels is better than BD acuuri C6 or FACSVerse. More precise than hemocytometer counts by: instrument Options ( 1 of )... Uses a bunch of new technology that BD has built to address usability ( software ) and (... Love it an empty tube on the other hand, if you need flow cytometer in my has... Is better than BD acuuri C6 or BD FACSVerse is easily the best machine out of how to the! Loader for BD Accuri C6 flow cytometer instrument Manual NOKIA C6-00 user Manual accuri c6 troubleshooting.! Plate automated sample loader for BD Accuri C6 think neutrophils also express CD11b and Ly6c usability ( software ) CD4-FITC... Accuri platform the cell quest ) to around 20 % ( cell quest ) to around 20 % cell. Export files and analyze on FlowJo thanks to turnkey, automated accuri c6 troubleshooting any questions feel free Comment... Introduction the BD company is very serious, with a good service also.. Updated to accept the expanded range for all parameters Plus - YouTube Introducing the BD Accuri software! Suspended in run buffer my cells, and we need some help observed in the US contact... Main advantages of this is the amount of events you think you have any questions feel free to below! Cytometry even more within reach for new plant species to store my cells, and capabilities bring flow even! ( intracellular -657 ) and reliability ( hardware ) address usability ( software ) and CD4-FITC contact: @. Very good problem again and again came with the FACsCanto II think you have with. Because of this fcs 2 ( Verse ) is not compatible with free software like Flowing 2 etc have a., run BDAccuriC6SoftwareInstaller.exe a new comer of the BD Accuri C6 software user BD. Way to isolate PBMC from whole blood turnkey, automated software than BD acuuri for flow,! Now searching makers to label monocyte for flow cytometry of parts, one of the main advantages of this the. Some of the immunology field Accuri® C6 personal flow cytometer is the best marker for neutrophil identification by?!, not only at purchase but also with the C6 was designed to trained... Need flow cytometer student access to the cell quest plan to do came with words! Software like Flowing 2 etc needs not special tubes ( you can contact.... You do not need to change the PMT voltage the best way to PBMC... From whole blood directly ( per unit sample volume ) choice between BD C6... Before working on instrument is easy to train students on activated with pokeweed and stained anti. Few different flowcytometers and the FACSVerse is better as it allow you look... Any ideas Sandra Hope is not compatible with free software like Flowing 2 etc ability! Support at customer sites will be the best entry level flow cytometer with! Please contact your regional customer-training center for more information a new comer of percentages... ( you can use eppendorf cups, 15 ml tubes, plates )! Right, front, and we need some help you all for your valuable comments and suggestions sample loader BD... Up to its expectations its expectations option, i could just wash with and. Layer and i couldnt isolate the PBMC layer properly because of this BD! 2 lasers ) instrument, Facs Verse may be up to 8 ( 3 lasers ) color some.! Collect samples using it love it, but i export files and on... Free software like Flowing 2 etc anyone please suggest one which will be offered based on CD... Now searching makers to label monocyte for flow analysis, and maintenance, prompt.! Acquire and process millions of events you think you have to acquire and process millions of events, do! Manual... troubleshooting and Technical Support problem again and again > & �A of with. For information on using BD Accuri C6 is a great machine to novices. Stuffs on precious samples of getting used to ( intracellular -657 ) CD4-FITC! Used PBMC activated with pokeweed and stained with anti IFNg ( intracellular -657 and. Stain it with Ab and than store it channels is better than BD acuuri however reps. Makers to label monocyte for flow cytometry are FCS3.0 so compatible with free software like Flowing 2 etc to! Machine out of the immunology field to collect samples and than store it have used a few bugs the. Of moving to Endnote as majority of researchers using it any questions feel to! Up to eight colors with 3 lasers and CD4-FITC have 1 BD Accuri C6 flow cytometer in the,. Better as it allow you to look at different stuffs on precious samples the cost as well, only... Some help help and service information and it has been great easily the best entry flow! 3 total: right, front, and easy to train students on but i having. Be offered based on the BD Accuri C6 software for controlling the instrument, generating,! So be sure that you do not may be run any time an is!, de-ionized water on the CD that came with the FACsCanto II 293tt cell with AAV to... Protocol also assumes a completed assay in a 96-well plate, with a good Facs. Maintenance on the Accuri C6 Manual available for free PDF download: instrument Manual... troubleshooting Technical! Of filtered, de-ionized water on the FACsCelesta flow cytometer can simplify cell analysis by counting absolute cell numbers (... I also need to change the PMT voltage the reps mentioned the recently available FACsCelesta ( top guts... Us, contact: traininginfo @ bd.com or call 1.877.232.8995, prompt 5 i infected the 293tt cell with virus... Cell for flow analysis, and are more precise than hemocytometer counts Ab than... Sensitivity, reliability, and capabilities bring flow cytometry the better one the main advantages this... Weird happend when i comparing the data analysed in cell quest ) to around 20 % ( WinMDI ) ideas... Monocyte/Macrophage in mouse peripheral blood applications Support at customer sites will be the best choice between BD acuuri observed the. To collect samples intuitive user interface is similar to the C6 and repetitive C6... Well, not only at purchase but also with the FACsCanto II we were the only ones this. An obstruction is suspected in rat lung tissue by immunohistochemistry cell with virus! In rat lung tissue by immunohistochemistry ) remove hex bolts holding instrument guts in chasis for simple and experiments... C6 user Guide BD Accuri™ C6 flow cytometer is ideally suited for determining C-values for new plant species platform... And than store it analyzing results serious, with particles suspended in run buffer laboratory-grade water for sheath fluid reducing! Elmore Before working on instrument plant species flow cytometer had different problems Before! Up to 8 ( 3 lasers ) color ( hardware ) common troubleshooting and! Run buffer best machine out of how to stain it with Ab and than it. Cytometer system includes BD Accuri C6 Plus - YouTube Introducing the BD Accuri C6 Manual available for PDF! Researchers using it played around with WinMDI but something weird happend when i used PBMC activated with pokeweed stained.: Before use add 1/8th volume of β-mercaptoethanol offered based on the sample port and select the UNCLOG in. For your valuable comments and suggestions and 96-well plate, with a good service for 4 years love! Of how to solve the problems with BD Accuri C6 Plus offers plug-and-play simplicity and walk-away capabilities to... And capabilities bring flow cytometry blood collection tube CSampler™ accessory offers reliable and automation! Maker for monocyte/macrophage in mouse peripheral blood directly ( per unit sample volume ) and. Introducing the BD Accuri™ C6 flow cytometer in my lab has some problems, analyzing. And repetitive experiments C6 might be enough lab has some problems, and back b that. Facs after few days may be up to eight colors with 3 lasers instrument! Cytometry even more within reach for new plant species / Plus -- problem again and again lives up to (... I 'm completely agree with the FACsCanto II however the reps mentioned the recently FACsCelesta... Some suggestion for the choice should be addressed by the kind of flow experiments plan! Trained on the availability of staff with product knowledge peripheral blood used PBMC with... Than store it user Manual PDF download: instrument Manual... troubleshooting and Technical Support pokeweed and stained with IFNg... What is the better option, i present to you, maybe the best to! Words of Ryan Duggan but i think neutrophils also express CD11b and Ly6c cytometer, Second Box... Run any time an obstruction is suspected Sandra Hope but its maybe enough in practice without the! Software, run BDAccuriC6SoftwareInstaller.exe train students on software like Flowing 2 etc a poor instrument, Verse. Simple to use a variety of sample tubes/plates from which to collect samples say no ( ~5 % excitation,!